Reference type

Thesis
 

Record Number

8

Author

Uruakpa, Uloma Adaeze

Year

1997

Title

Preservation of Newcastle disease vaccine at room temperature

Academic Department:

Veterinary Pathology

City

Brisbane, Australia

University

University of Queensland

Thesis Type:

M.V.Sc.

Label

Thesis

Keywords

Australia, Cell culture, I-2 vaccine, V4 vaccine

Notes

The growth of the Australian Newcastle disease vaccines strain V4 and strain I-2 was studied in cell culture. I-2 virus was cytopathic in cultures of chick embryo fibroblasts, kidney cells and liver cells. V4 produced cytopathic changes only in the liver cells. A batch of I-2 vaccine was prepared under conditions simulating those that pertain in regional laboratories in developing countries. Vaccine was grown in embryonated eggs from a free-range flock that were incubated in a simple incubator. Vaccine was stored in liquid form in 1% gelatin. There was only a slight loss in titre on storage at 22oC for 2 weeks.